Wednesday, December 17, 2008

Chapter 45 and 46 Questions Due January 6th!!!

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Chapter 45 Questions

1.What is a population?
2.What are four characteristics of populations?
3.Describe the three patterns that populations can be dispersed in:
a. clumped b. uniform c. random
4. How can you determine the number of animals in a particular area?
5. What four factors affect population size?
6. What is zero population growth?
7. What is the growth rate formula?
8. What is biotic potential?
9. Name three parameters which influence why biotic potential varies from species to species.
10. Explain the three limits of growth on populations.
a. Density dependent
b. Carrying capacity and logistic growth
c. Density Independent limit
11. What is a life table?
12. What is a survivorship curve? Explain Type I, Type II, and Type III
13. What factors affected human population growth? (Name 3)
14. What is total fertility rate?
15. How does age structure influence population growth?
16. Describe the four stages of the demographic transition model:
a. preindustrial
b. transitional
c. industrial
d. postindustrial
17. What % of the world’s goods does the US use? What % of the population does the US represent?

Chapter 46 Questions

1. Define the following ecological terms: habitat, niche, community, symbiotic, competition, predation, parasitism, and mutualism.
2. What five factors shape a community?
3. Compare and contrast facultative and obligatory mutualism.
4. Compare and contrast interspecific and intraspecific competition.
5. Explain the theory of competitive exclusion.
6. Explain the Type I, Type II, and Type III models of Predator/Prey interactions.
7. Explain the relationship between the Canadian lynx and snowshoe hare.
8. Discuss four types of prey defenses.
9. What are parasites and parasitoids.
10. Discuss five attributes that make parasites and parasitoids good controls.
11. Explain the relationship between the cowbirds and the buffalo.
12. Explain the following terms: ecological succession, pioneer species, primary and secondary succession.
13. What are key stone species?
14. Name three ways geographic dispersal of species can occur.
15. What has been the result of the following exotic species?
a. alga Caulerpa
b. Kudzu
c. Rabbits in Autralia
16. What happens to the number of species as you move from the Arctic to temperate to tropical zones?
17. Name three reasons biodiversity is favored in the tropics.
18. Compare and contrast distance and area affect of islands.

Monday, December 8, 2008

AP Lab #6 Outline: Molecular Genetics

Title: AP Lab #6 Molecular Genetics

Introduction

1st paragraph: Purpose of lab - understand basic principles of genetic engineering -In the first part of the lab, you will use antibiotic-resistance plasmids to transform Escherichia coli. In the second part, you will use gel electrophoresis to separate fragments of DNA for further analysis.

2nd paragraph: Discuss background information about bacterial transformation - define transformation and discuss how a plasmid is used to insert foreign DNA into a host organism.

3rd paragraph: Discuss background information about gel electrophoresis - restriction enzymes - where they come from? Hae III and EcoRI - sticky vs. blunt cuts - the principles of gel electrophoresis - charges - positive and negative ends - purpose - to determine sizes of cut DNA fragments.

4th paragraph: Briefly discuss procedure of Part 1 & Part 2 - Part 1 we had controls and experimental colonies of E.coli. In the control we inserted no plasmids and in the experimental group we inserted a plasmid which included a gene for antibiotic resistance. E.coli typically do not duplicate in the presence of ampicillin. Cultures were grown on 5 plate, two plates of agar with the plasmid and without the plasmid, two plates of ampicillin - with and without the plasmid, one plate with ampicillin and pBlu which would make the E.coli blue when they digested the agar. This plate had E.coli with the plasmid. Predictions were made about which colonies would and would not survive on each plate and results were calculated. In Part 2 two restriction enzymes were used to cut DNA and compared to an uncut sample of DNA. Hae III and EcoRI were used to cut DNA and compared to uncut DNA. They underwent gel elecrophoresis. Bands were stained and measured. A logarithmic graph was used to determine the number of base pairs each DNA fragment was made up of.

5th paragraph: Relate this lab to one of the major themes of AP Biology:

1. Science as a Process

2. Evolution

3. Energy Transfer

4. Continuity and Change

5. Relationship of Structure to Function

6. Regulation

7. Interdependence in Nature

8. Science, Technology, and Society

Procedure

Summarize the Procedure for Part 1: Bacterial Transformation
(if you do not have this lab sheet see me I have many copies)

Summarize the Procedure for Part 2: Gel Electrophoresis

Data

One to three sentence summary of Part 1:

Table 1: Results of Bacterial Transformation

Agar Plate with No Plasmid - Growth or No Growth
Agar Plate with Plasmid - Growth or No Growth
Agar Plate with Ampicillin and with No Plasmid - Growth or No Growth
Agar Plate with Ampicillin and Plasmid - Growth or No Growth
Agar Plate with Ampicillin and pBlu and Plasmid - Growth or No Growth

One to three sentences summary of Part 2:

Table 2: Hae III Fragment Sizes
(Table 6.1 we did in class)

Table 3: EcoRI Fragment Sizes
(Table 6.2 we did in class)

Graph 1: Fragment Sizes made from Restriction Enzymes
(Use Log Graph we did in class)

Conclusion

Paragraph 1: Summarize Purpose of two parts of lab, procedures, and predictions.

Paragraph 2: Summarize Results from Part 1: What were the results of the transformations? What was the transformation efficiency?

Paragraph 3: Summarize the Results for Part 2: What were the results of gel electrophoresis? How were fragment sizes determined? How did your interpolated results compare to actual results?

Paragraph 4: Discuss errors in lab

Paragraph 5: Discuss improvements to lab

Paragraph 6: Relate this lab to major themes in AP Biology

Tuesday, December 2, 2008

Retest Opportunities

For those of you who are interested in doing a re-test for the Ch. 13 & 14 test
or Ch. 15 & 16 test you can do this by:

Ch. 13 & 14: Answer Critical Thinking Questions on page 217 #'s 1, 2, 3, 4 and on page 229 #'s 1,2,5,& 6

Ch. 15 & 16: Answer Critical Thinking Questions on page 241 #'s 2, 6,8 and 258 #'s 1 & 3

Chapter 17 Reading Questions

These questions will be due Monday December 8th!

Questions here

Schedule for Thursday December 4th - Friday December 12th

Thursday - Part II of AP Bio Lab #6 ( You will be given a study packet for the final)
We will spend about 15 min. of each class going over study questions. The final will be
AP exam questions for the topics we have covered. You will be given a packet of short readings
and practice multiple choice questions for the units we have covered. They are:
1. Chemistry
2. Cells
3. Respiration
4. Photosynthesis
5. Cell Division
6. Heredity
7. Molecular Genetics
8. Labs - (1 - diffusion and osmosis, 2 - enzyme catalysis, 3 - mitosis and meiosis, 4 - plant pigments and photosynthesis, 5 - cell respiration, 6 - molecular biology, 11- animal behavior)
Friday - Lab Analysis and introduction to Evolution

Exam will be two parts - Part 1: Friday 12/12 - 3 essay questions
Wednesday 12/17 - 120 multiple choice questions

Monday - Chapter 17 Questions due & Review for Final
Tuesday - Review for Final
Thursday - Review for Final - AP Lab #6 Due
Friday - Part 1 of Final - 3 essay questions

Monday, December 1, 2008

Lac Operon Animation & Essay Question

Your take home essay for the Chapter 15 & 16 question is about the lac operon.

Below is an animation to help you with your understanding of this type of control:

http://highered.mcgraw-hill.com/olc/dl/120080/bio27.swf

Essay Question due Tuesday 12/2

Describe the operon hypothesis and discuss how it explains the control of messenger RNA production and the regulation of protein synthesis in bacterial cells.